Evaluation of antioxidant activity of unpurified and purified datura seed

Aim: Evaluation of effect shodhana process datura seed (Datura stramonium) antioxidant activity. Methods: The was purified with two different processes viz. cow’s urine, and milk by Dolayantra method. Antioxidant activity evaluated in-vitro 1,1-Diphenyl-2-picryl hydrazyl (DPPH) radicals scavenging Ferric reducing power ability (FRPA) assay. Result: cow urine (CUD) showed a significant in inhibiting DPPH, reaching up to 85.96% at concentration 1000 mcg/ml its IC50 314.3 while Unpurified Datura Seed (UD) 86.06% 171.73 mcg/ml. Cow Milk Purified (CMD) extract reach 69.72% value 640. Ascorbic acid 23.35 CUD reduction ferric ion, 85.59% EC50 304.46 UD 86.11% 280.28 CMD 52.28% 1091.3mcg/ml.